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Colour deconvolution specifications


Unique identifier OMICS_25612
Name Colour deconvolution
Software type Application/Script
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Programming languages Java, Shell (Bash)
License GNU General Public License version 3.0
Computer skills Advanced
Version 3.0.2
Stability Stable
Maintained Yes




No version available

Colour deconvolution citations


Connective tissue growth factor specific monoclonal antibody inhibits growth of malignant mesothelioma in an orthotopic mouse model

PMCID: 5915087
PMID: 29719620
DOI: 10.18632/oncotarget.24892
call_split See protocol

[…] cal specimens stained by light green were digitized, using the microscope and camera described above. We analyzed the image using the ImageJ software and color deconvolution plugin ( for ImageJ, and Fiji implements staining separation by the method of Ruifrok and Johnston []. Extracted light green-positive area was converted to black (threshold: upper cutoff, […]


Differential spatial expression of peripheral olfactory neuron derived BACE1 induces olfactory impairment by region specific accumulation of β amyloid oligomer

PMCID: 5596540
PMID: 28796251
DOI: 10.1038/cddis.2017.349
call_split See protocol

[…] he OB from three tissue sections per animal. To quantify the reciprocal intensity, by measuring the intensity per unit area, with ImageJ, using the color deconvolution plug-in (, The target unit area of images processed using the color deconvolution tool in imageJ to separate brown from other color in images. The area of brown staining was then quantif […]


PKM2 promotes tumor angiogenesis by regulating HIF 1α through NF κB activation

Mol Cancer
PMCID: 4704385
PMID: 26739387
DOI: 10.1186/s12943-015-0490-2

[…] est. p < 0.05 or less was considered significant. The quantitative analysis of immunohistology staining was performed with Image J software (National Institute of Health, Bethesda, MD, USA) using the Colour Deconvolution plugin. […]


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Colour deconvolution institution(s)
Department of Pathology, M.D. Anderson Cancer Center, Houston, TX, USA

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