Conventional fluorescence microscopy analysis software tools | Bioimaging
The wide-field fluorescence microscope is a modification of the compound optical microscope, arranged to admit light of a selected wavelength to fluorescently labeled specimens and to image them through the emitted radiation. Since fluorescence emission has a lifetime in the nanosecond scale, continuous excitation is needed to collect an image having fluorescence as contrast mechanism. In a classical wide-field optical fluorescence microscope, the light source of a fluorescent microscope is a gas-vapor arc lamp, using either a discrete (Mercury) or a continuous (Xenon) light-emission spectrum.
(Diaspro et al., 2013) Optical Fluorescence Microscopy. Encyclopedia of biophysics.