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Citations per year

Number of citations per year for the bioinformatics software tool CRISPOR
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Protocols

CRISPOR specifications

Information


Unique identifier OMICS_12490
Name CRISPOR
Interface Web user interface
Restrictions to use None
Input data A single genomic sequence (< 1000 bp), a genome, a protospacer adjacent motif (PAM)
Computer skills Basic
Version 3.1
Stability Stable
Source code URL http://crispor.tefor.net/downloads/
Maintained Yes

Documentation


Maintainer


  • person_outline Maximilian Haeussler

Information


Unique identifier OMICS_12490
Name CRISPOR
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Computer skills Advanced
Stability Stable
Maintained Yes

Versioning


No version available

Documentation


Maintainer


  • person_outline Maximilian Haeussler

Publications for CRISPOR

CRISPOR citations

 (28)
call_split

Genome wide prediction of CRISPR/Cas9 targets in Kluyveromyces marxianus and its application to obtain a stable haploid strain

2018
Sci Rep
PMCID: 5943413
PMID: 29743485
DOI: 10.1038/s41598-018-25366-z
call_split See protocol

[…] DMKU 3-1042 and NBRC1777 genomes as these two genomes were better annotated than the remaining six genomes. The genomes of DMKU 3-1042 and NBRC1777 were then integrated into the standalone version of CRISPOR, which was then used to predict the gRNA sequences with NGG PAM by using the Matα3 gene sequence as the query. As the gene sequences of Matα3 and HMLα3 are identical, we selected the gRNAs tha […]

library_books

Locus specific engineering of tandem DNA repeats in the genome of Saccharomyces cerevisiae using CRISPR/Cas9 and overlapping oligonucleotides

2018
Sci Rep
PMCID: 5940919
PMID: 29740109
DOI: 10.1038/s41598-018-25508-3

[…] The CRISPOR tool (http://crispor.tefor.net/) was used to select guide RNA sequences for targeting the YMR262 locus without any off-target (Supplementary Table ). To create the guide RNA expression plasmid […]

library_books

Precise Excision of the CAG Tract from the Huntingtin Gene by Cas9 Nickases

2018
Front Neurosci
PMCID: 5834764
PMID: 29535594
DOI: 10.3389/fnins.2018.00075

[…] In silico analysis using the CRISPOR tool (Haeussler et al., ) predicted 13 exonic off-target sites for HTT_sgRNA1 (with score > 10.00), 18 sites for HTT_sgRNA3 and 196 for HTT_sgRNA4. Notably, more than 98% of the exonic HTT_sgR […]

library_books

Multiplex CRISPR/Cas9 system impairs HCMV replication by excising an essential viral gene

2018
PLoS One
PMCID: 5813945
PMID: 29447206
DOI: 10.1371/journal.pone.0192602

[…] gene close to the start codon and at the beginning and end of the exon 5 to target all the IE splice variants (). Therefore, the sequence of exons 2 and 5 of the UL122/123 gene were entered into the CRISPOR software (http://crispor.tefor.net) and the human genome was used as a reference for the calculation of the off-target sites. Fourteen different HCMV genome sequences available at NCBI were al […]

library_books

Molecular biology at the cutting edge: A review on CRISPR/CAS9 gene editing for undergraduates

2018
Biochem Mol Biol Educ
PMCID: 5901406
PMID: 29381252
DOI: 10.1002/bmb.21108

[…] e, however efficiency for inducing a desired mutation or edit is inversely correlated to the number of base pairs from a PAM site . To facilitate guide RNA design, CRISPR design tools, such as http://crispor.tefor.net/, scan the specificity of a target sequence to minimize off‐target effects. If target sequences are not specific enough, Cas9 can bind and cut in a different place than intended and […]

library_books

Unbiased compound protein interface mapping and prediction of chemoresistance loci through forward genetics in haploid stem cells

2018
Oncotarget
PMCID: 5839405
PMID: 29515774
DOI: 10.18632/oncotarget.24305

[…] smb5 mutations were engineered in WT haploid embryonic stem cells using the CRISPR/Cas9 technology as previously described []. DNA template sequences for small guide RNAs were designed online (http://crispor.org), purchased from Sigma and cloned into the Cas9-GFP expressing plasmid PX458 (addgene #48138, ). Corresponding guide and Cas9 expressing plasmids were cotransfected with the respective sin […]


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CRISPOR institution(s)
Santa Cruz Genomics Institute, MS CBSE, University of California, Santa Cruz, CA, USA; Central Institute of Mental Health, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany; Institut Curie, CNRS UMR3215, INSERM U934, Paris, France; CNRS UMR 7622, INSERM U1156, Sorbonne Université Paris 06, Paris, France; Mary Lyon Centre, MRC Harwell, Didcot, UK; TEFOR Infrastructure, Gif-sur-Yvette, France; INSERM U1154, CNRS UMR 7196, Muséum National d’Histoire Naturelle, Paris, France

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