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CRISPOR specifications

Information


Unique identifier OMICS_12490
Name CRISPOR
Interface Web user interface
Restrictions to use None
Input data A single genomic sequence (< 1000 bp), a genome, a protospacer adjacent motif (PAM)
Computer skills Basic
Version 3.1
Stability Stable
Source code URL http://crispor.tefor.net/downloads/
Maintained Yes

Documentation


Maintainer


  • person_outline Maximilian Haeussler <>

Information


Unique identifier OMICS_12490
Name CRISPOR
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Computer skills Advanced
Stability Stable
Maintained Yes

Versioning


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Documentation


Maintainer


  • person_outline Maximilian Haeussler <>

Publications for CRISPOR

CRISPOR in publications

 (26)
PMCID: 5943413
PMID: 29743485
DOI: 10.1038/s41598-018-25366-z

[…] 3-1042 and nbrc1777 genomes as these two genomes were better annotated than the remaining six genomes. the genomes of dmku 3-1042 and nbrc1777 were then integrated into the standalone version of crispor, which was then used to predict the grna sequences with ngg pam by using the matα3 gene sequence as the query. as the gene sequences of matα3 and hmlα3 are identical, we selected the grnas […]

PMCID: 5940919
PMID: 29740109
DOI: 10.1038/s41598-018-25508-3

[…] length (4, 46 or 165 bp), oligonucleotide combinations were designed in order to create g/c-poor (25%g/c), gc-neutral (50%g/c) or g/c-rich (75% g/c) tandem dna repeats of arbitrary sequence., the crispor tool (http://crispor.tefor.net/) was used to select guide rna sequences for targeting the ymr262 locus without any off-target (supplementary table ). to create the guide rna expression […]

PMCID: 5834764
PMID: 29535594
DOI: 10.3389/fnins.2018.00075

[…] ariaiii) 48 h post electroporation and collected for genomic dna, rna and protein extraction., guide rna sequences for the crispr/cas9 system were designed as described in ran et al. with the use of crispor software (http://crispor.tefor.net/crispor.py; haeussler et al., ). briefly, the top and bottom strand of 20-nt guide rna were synthesized (ibb, warsaw), annealed and ligated into the pair […]

PMCID: 5813945
PMID: 29447206
DOI: 10.1371/journal.pone.0192602

[…] gene close to the start codon and at the beginning and end of the exon 5 to target all the ie splice variants (). therefore, the sequence of exons 2 and 5 of the ul122/123 gene were entered into the crispor software (http://crispor.tefor.net) and the human genome was used as a reference for the calculation of the off-target sites. fourteen different hcmv genome sequences available at ncbi […]

PMCID: 5839405
PMID: 29515774
DOI: 10.18632/oncotarget.24305

[…] mutations were engineered in wt haploid embryonic stem cells using the crispr/cas9 technology as previously described []. dna template sequences for small guide rnas were designed online (http://crispor.org), purchased from sigma and cloned into the cas9-gfp expressing plasmid px458 (addgene #48138, ). corresponding guide and cas9 expressing plasmids were cotransfected with the respective […]


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CRISPOR institution(s)
Santa Cruz Genomics Institute, MS CBSE, University of California, Santa Cruz, CA, USA; Central Institute of Mental Health, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany; Institut Curie, CNRS UMR3215, INSERM U934, Paris, France; CNRS UMR 7622, INSERM U1156, Sorbonne Université Paris 06, Paris, France; Mary Lyon Centre, MRC Harwell, Didcot, UK; TEFOR Infrastructure, Gif-sur-Yvette, France; INSERM U1154, CNRS UMR 7196, Muséum National d’Histoire Naturelle, Paris, France

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