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CRISPR Assembler Crass

Identifies and reconstructs CRISPR loci from raw metagenomic data without the need for assembly or prior knowledge of CRISPR in the data set. CRISPR in assembled data are often fragmented across many contigs/scaffolds and do not fully represent the population heterogeneity of CRISPR loci. Crass identified substantially more CRISPR in metagenomes previously analysed using assembly-based approaches. The increased sensitivity, specificity and speed of Crass will facilitate comprehensive analysis of CRISPRs in metagenomic data sets, increasing our understanding of phage-host interactions and co-evolution within microbial communities.

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Crass forum

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Crass versioning

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Crass classification

  • Archaebacteria
  • Eubacteria

Crass specifications

Software type:
Package/Module
Restrictions to use:
None
Operating system:
Unix/Linux
Computer skills:
Advanced
Maintained:
Yes
Interface:
Command line interface
Output data:
Crass outputs a standard xml file that describes all of the CRISPRs that it discovers. This file format is open source and is being developed to facilitate the interchange of CRISPR data between different research groups.
License:
GNU General Public License version 2.0
Stability:
Stable

Crass support

Documentation

Maintainer

  • Gene W. Tyson <>

Credits

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Publications

Institution(s)

Australian Centre for Ecogenomics, School of Chemistry and Molecular Biosciences; Advanced Water Management Centre, The University of Queensland, St Lucia, Brisbane, Queensland, Australia

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