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A database of CRISPR/Cas9 target sequences that have been experimentally validated in zebrafish. CRISPRz can be searched using multiple inputs such as ZFIN IDs, accession number, UniGene ID, or gene symbols from zebrafish, human and mouse. CRISPRz was developed in an effort to provide a comprehensive list of validated CRISPR targets from published sources as well as from an ongoing genome-wide knockout project in the zebrafish genome. Data will be added as more validated CRISPR targets are published or contributed from unpublished, in-house projects. The database is also open for data submission from the research community.
WGE / Wellcome Trust Sanger Institute Genome Editing database
Uses methods to compute, visualize and select optimal CRISPR sites in a genome browser environment. The WGE database currently stores single and paired CRISPR sites and pre-calculated off-target information for CRISPRs located in the mouse and human exomes. Scoring and display of off-target sites is simple, and intuitive, and filters can be applied to identify high-quality CRISPR sites rapidly. WGE also provides a tool for the design and display of gene targeting vectors in the same genome browser, along with gene models, protein translation and variation tracks.
Utilizes the CRISPRFinder program to identify putative CRISPRs and additional tests to further screen for the smallest CRISPRs in a polyphasic approach. Indeed the CRISPRFinder program is conceived to authorize the largest number of possible CRISPRs, especially the shortest ones, containing one or two spacers. The main idea of the program is to first find possible CRISPR localizations in a genomic sequence and then check if these regions contain a cluster that possess the characteristics of "obvious" CRISPR, i.e. containing at least three repeats.
Facilitates the use of the CRISPR/Cas9 system as a genome editing tool for functional studies and molecular breeding of grapes. Among other functions, the Grape-CRISPR database allows users to identify and select multi-protospacers for editing similar sequences in grape genomes simultaneously. The database contains two main sections: Search and Design. In the Search section, users can identify appropriate protospacer and protospacer-adjacent motif (PAM) sites of a gene by providing certain inquiry information such as locus location, gene ID or Pfam ID. The Design section is for protospacer design. Users can detect and design protospacers and PAMs in the sequences of interest by using the Perl scripts provided.
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