CRISPR design statistics

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Associated diseases

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CRISPR design specifications


Unique identifier OMICS_06185
Name CRISPR design
Interface Web user interface
Restrictions to use None
Input data Target region seq (single and batched)
Computer skills Basic
Stability Stable
Maintained Yes


  • person_outline Feng Zhang <>

Publication for CRISPR design

CRISPR design in publications

PMCID: 5912780
PMID: 29684067
DOI: 10.1371/journal.pone.0196238

[…] computational data analysed in this study were collected from open access sources, as detailed in the appropriate sections., target sequences () for sgrna design were chosen using the online based crispr design tool (, chopchop.v1 or v2 [,] or crisprscan []. target site uniqueness was verified with the ncbi blast analysis against the zebrafish genome (grcz10). sgrnas […]

PMCID: 5913311
PMID: 29686284
DOI: 10.1038/s41467-018-03900-x

[…] lentiviral infection efficacy was >90% and cells were maintained with 8 μg/ml puromycin. multiple synthetic guide rnas (grnas) (crispr crrna, integrated dna technologies) were designed using the crispr design tool (, those with off-target effects were excluded. grnas were delivered by transient transfection reagent transit-x2 (mirus bio). partial pten knockout was confirmed […]

PMCID: 5947990
PMID: 29638216
DOI: 10.7554/eLife.35069.034

[…] as a standalone script retrieved from the ‘sgrna designer’ website ( and the latter was implemented as documented on the ‘crispr design’ website ( off targets for each crrna were found using the short read aligner tool bowtie, searching up to three mismatches (). off targets that then match […]

PMCID: 5738113
PMID: 29261807
DOI: 10.1371/journal.ppat.1006758

[…] systems) respectively with the bio-rad chemidoc xrs gel documentation system (bio-rad laboratories)., crispr-cas9 single guide rnas (sgrnas) for selected target genes were designed using the crispr design tool [], sgrna designer [] and e-crispr v4.2 [] online tools based on the target genome sequences obtained from ensembl genome browser or merged ensembl/havana transcripts. […]

PMCID: 5727232
PMID: 29234012
DOI: 10.1038/s41467-017-02054-6

[…] with respect to 1h. nmrpipe and mddnmr were used for spectra processing and spectra assignment and analysis was performed with cara., sgrnas targeting genomic regions of interest were designed using crispr design tool ( and synthesized by idt, inc. single cells were sorted onto irradiated mef feeder for increased viability through facs 72 h post transfection. mutant clones […]

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CRISPR design institution(s)
Broad Institute of MIT and Harvard, Cambridge, MA, USA; McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA

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