CRISPR-DO statistics

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Associated diseases

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CRISPR-DO specifications

Information


Unique identifier OMICS_12378
Name CRISPR-DO
Interface Web user interface
Restrictions to use None
Output data The output of CRISPR-DO contains a table including information and scores for each sgRNA.
Programming languages Python
Computer skills Basic
Version 0.1
Stability Stable
Maintained Yes

Taxon


  • Invertebrates
    • Caenorhabditis elegans
    • Drosophila melanogaster
  • Primates
    • Homo sapiens
  • Rodents
    • Mus musculus
  • Vertebrates
    • Danio rerio

Documentation


Maintainer


  • person_outline Liu Shirley <>

Information


Unique identifier OMICS_12378
Name CRISPR-DO
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Programming languages Python
Computer skills Advanced
Stability Stable
Requirements
bx-Python, tabix, SSC
Maintained Yes

Taxon


  • Invertebrates
    • Caenorhabditis elegans
    • Drosophila melanogaster
  • Primates
    • Homo sapiens
  • Rodents
    • Mus musculus
  • Vertebrates
    • Danio rerio

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Documentation


Maintainer


  • person_outline Liu Shirley <>

Publication for CRISPR-DO

CRISPR-DO in publications

 (2)
PMCID: 5898912
PMID: 29565247
DOI: 10.7554/eLife.35574.023

[…] applications. first, ~90% of insertions in essential genes cause a severe loss-of-function phenotype, an effective way to mutagenize genes. interestingly, 12/14 chromosomes engineered through crispr do not carry second-site lethal mutations. second, 26/36 (70%) of lethal insertions tested are rescued with a single uas-cdna construct. third, loss-of-function phenotypes associated with many […]

PMCID: 2886076
PMID: 20559554
DOI: 10.1371/journal.pone.0011126

[…] that spacers are chronological records reflecting previous encounters with mobile genetic elements . however, the loss of one or more repeat-spacer units has been observed. this suggests that crispr do not grow unchecked. one would assume that older spacers should be more frequently deleted because they have been inserted for a longer time. not only older spacers had longer opportunity […]


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CRISPR-DO institution(s)
School of Life Science and Technology, Tongji University, Shanghai, China; Department of Biostatistics and Computational Biology, Dana-Farber Cancer Institute and Harvard School of Public Health, Boston, MA, USA; Center for Functional Cancer Epigenetics, Dana-Farber Cancer Institute, Boston, MA, USA; Division of Molecular and Cellular Oncology, Department of Medical Oncology, Dana-Farber Cancer Institute and Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA, USA; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
CRISPR-DO funding source(s)
The project was partially supported by the National Natural Science Foundation of China [31329003], NIH R01 HG008728, and the Claudia Adams Barr Award in Innovative Basic Cancer Research from Dana-Farber Cancer Institute.

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