CRISPR-GA specifications

Information


Unique identifier OMICS_05089
Name CRISPR-GA
Alternative name CRISPR Genome Analyzer
Interface Web user interface
Restrictions to use None
Input data The user is only required to use polymerase chain reaction (PCR) to amplify the area of the genome to analyze. This amplified library is sequenced with an Illumina Miseq Machine (other platforms may also be used). The user provides to the pipeline CRISPR-GA, the next-generation sequencing obtained reads, the intended editing sequence and the original sequence.
Output data CRISPR-GA will estimate the HR (normally associated with intended edits), non-homologous end-joining (NHEJ, normally associated with non-desired edits) and a complete report of the location and characteristics of the indels.
Computer skills Basic
Stability Stable
Maintained Yes

Maintainer


  • person_outline George M. Church <>

Publication for CRISPR Genome Analyzer

CRISPR-GA institution(s)
Department of Genetics, Harvard Medical School, Boston, MA, USA
CRISPR-GA funding source(s)
CEGS grant (Centers of Excellence in Genomic Science, grant number HG005550, National Institutes of Health); a Human Frontiers Science Program Long Term Fellowship

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