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CRISPR Genome Analyzer CRISPR-GA

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A platform to assess the quality of a genome editing experiment only with three mouse clicks. The method evaluates next-generation data to quantify and characterize insertions, deletions and homologous recombination. CRISPR Genome Analyzer provides a report for the locus selected, which includes a quantification of the edited site and the analysis of the different alterations detected. The platform maps the reads, estimates and locates insertions and deletions, computes the allele replacement efficiency and provides a report integrating all the information.

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CRISPR-GA classification

CRISPR-GA specifications

Interface:
Web user interface
Input data:
The user is only required to use polymerase chain reaction (PCR) to amplify the area of the genome to analyze. This amplified library is sequenced with an Illumina Miseq Machine (other platforms may also be used). The user provides to the pipeline CRISPR-GA, the next-generation sequencing obtained reads, the intended editing sequence and the original sequence.
Computer skills:
Basic
Maintained:
Yes
Restrictions to use:
None
Output data:
CRISPR-GA will estimate the HR (normally associated with intended edits), non-homologous end-joining (NHEJ, normally associated with non-desired edits) and a complete report of the location and characteristics of the indels.
Stability:
Stable

CRISPR-GA support

Maintainer

  • George M. Church <>

Credits

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Publications

Institution(s)

Department of Genetics, Harvard Medical School, Boston, MA, USA

Funding source(s)

CEGS grant (Centers of Excellence in Genomic Science, grant number HG005550, National Institutes of Health); a Human Frontiers Science Program Long Term Fellowship

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