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CRISPR gRNA Design tool specifications

Information


Unique identifier OMICS_06187
Name CRISPR gRNA Design tool
Interface Web user interface
Restrictions to use None
License Commercial
Computer skills Basic
Stability Stable
Maintained No

Maintainer


This tool is not maintained anymore.

CRISPR gRNA Design tool citations

 (9)
library_books

The Apicomplexa specific glucosamine 6 phosphate N acetyltransferase gene family encodes a key enzyme for glycoconjugate synthesis with potential as therapeutic target

2018
Sci Rep
PMCID: 5838249
PMID: 29507322
DOI: 10.1038/s41598-018-22441-3

[…] oval from the Comitè Ètic Investigació Clínica Hospital Clínic de Barcelona. A single guide RNA (sgRNA) targeting the PfGNA1 consensus motif [(R/Q)-X-X-Q-X-G] was chosen using the Eukaryotic Pathogen CRISPR gRNA Design Tool. For PfGNA1-disruption homology regions (HR) 1 and 2 were amplified from 3D7 P. falciparum genomic DNA using primers P1/P2 and P3/P4 and cloned in plasmid pL7 using SpeI/AflII […]

library_books

A peptide tag specific nanobody enables high quality labeling for dSTORM imaging

2018
Nat Commun
PMCID: 5834503
PMID: 29500346
DOI: 10.1038/s41467-018-03191-2

[…] Paired sgRNAs were designed for the ACTB (actin beta, Homo sapiens; PubMed Gene ID: 60) target gene locus using an online CRISPR gRNA design tool, and synthesized as Ultramer DNA Oligonucleotides (Integrated DNA Technologies), ACTB_sgRNA and ACTB_HDR (Supplementary Table ).Next, paired sgRNAs were cloned according to a […]

library_books

Characterisation of Casein Kinase 1.1 in Leishmania donovani Using the CRISPR Cas9 Toolkit

2017
Biomed Res Int
PMCID: 5733176
PMID: 29333442
DOI: 10.1155/2017/4635605

[…] genome (PRJNA396645, https://www.ncbi.nlm.nih.gov/bioproject/396645) to design the primers required to generate the donor DNA and the corresponding sgRNAs. Sequences were identified using the EuPaGDT CRISPR gRNA Design Tool [] with similar target parameters as those used in the LeishGEdit strategy []. LdB pTB007 promastigotes were then transfected with the two PCR fragments. The transgenic parasit […]

library_books

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR concatemer

2017
PMCID: 5612278
PMID: 28745625
DOI: 10.3791/55916

[…] tion is to explain how to opt for the best targeting strategy and how to design gRNAs containing specific overhangs for the CRISPR-concatemer vector.Design gRNAs against the genes of interest using a CRISPR gRNA design tool of choice. See the Table of Materials for an example. NOTE: When targeting a pair of paralogous genes, although it is possible to design one gRNA per gene, it is advisable to d […]

library_books

Distinct populations of inflammatory fibroblasts and myofibroblasts in pancreatic cancer

2017
PMCID: 5339682
PMID: 28232471
DOI: 10.1084/jem.20162024

[…] hermo Fisher Scientific) to create PSC lines stably expressing Cas9. Short guide RNAs (sgRNAs) against IL-6 (5′-CACCTATACCACTTCACAAGTCGG-3′ and 5′-CACCTAAGCCTCCGACTTGTGAAG-3′) were designed using the CRISPR GRNA Design Tool (Atum) and cloned into the LRG plasmid (Lenti-sgRNA-EFS-GFP; Addgene; ). Next, LRG lentivirus was produced in 293T cells, concentrated using Lenti-X Concentrator (Takara Bio In […]

library_books

Opposing Transcriptional Mechanisms Regulate Toxoplasma Development

2017
PMCID: 5322347
PMID: 28251183
DOI: 10.1128/mSphere.00347-16

[…] For disruption of ApiAP2 factors, we used the multi-guide-RNA (multi-gRNA) CRISPR-Cas9 system. The gRNA for each gene was generated by the CRISPR gRNA design tool supported by DNA2.0 (ATUM, Newark, CA). The CRISPR-Cas9 gRNA plasmid (pSAG1::Cas9-U6::sgUPRT) was provided by David Sibley (Washington University, St. Louis, MO). The single gR […]

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