CRISPR gRNA Design tool statistics

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CRISPR gRNA Design tool specifications

Information


Unique identifier OMICS_06187
Name CRISPR gRNA Design tool
Interface Web user interface
Restrictions to use None
License Commercial
Computer skills Basic
Stability Stable
Maintained No

Maintainer


This tool is not maintained anymore.

CRISPR gRNA Design tool in publications

 (9)
PMCID: 5838249
PMID: 29507322
DOI: 10.1038/s41598-018-22441-3

[…] from the comitè ètic investigació clínica hospital clínic de barcelona. a single guide rna (sgrna) targeting the pfgna1 consensus motif [(r/q)-x-x-q-x-g] was chosen using the eukaryotic pathogen crispr grna design tool. for pfgna1-disruption homology regions (hr) 1 and 2 were amplified from 3d7 p. falciparum genomic dna using primers p1/p2 and p3/p4 and cloned in plasmid pl7 using spei/aflii […]

PMCID: 5834503
PMID: 29500346
DOI: 10.1038/s41467-018-03191-2

[…] previously cleaned with a 2% solution of hellmanex iii (helma analytics)., paired sgrnas were designed for the actb (actin beta, homo sapiens; pubmed gene id: 60) target gene locus using an online crispr grna design tool, and synthesized as ultramer dna oligonucleotides (integrated dna technologies), actb_sgrna and actb_hdr (supplementary table )., next, paired sgrnas were cloned according […]

PMCID: 5733176
PMID: 29333442
DOI: 10.1155/2017/4635605

[…] (prjna396645, https://www.ncbi.nlm.nih.gov/bioproject/396645) to design the primers required to generate the donor dna and the corresponding sgrnas. sequences were identified using the eupagdt crispr grna design tool [] with similar target parameters as those used in the leishgedit strategy []. ldb ptb007 promastigotes were then transfected with the two pcr fragments. the transgenic […]

PMCID: 5612278
PMID: 28745625
DOI: 10.3791/55916

[…] is to explain how to opt for the best targeting strategy and how to design grnas containing specific overhangs for the crispr-concatemer vector., design grnas against the genes of interest using a crispr grna design tool of choice. see the table of materials for an example. note: when targeting a pair of paralogous genes, although it is possible to design one grna per gene, it is advisable […]

PMCID: 5339682
PMID: 28232471
DOI: 10.1084/jem.20162024

[…] fisher scientific) to create psc lines stably expressing cas9. short guide rnas (sgrnas) against il-6 (5′-cacctataccacttcacaagtcgg-3′ and 5′-cacctaagcctccgacttgtgaag-3′) were designed using the crispr grna design tool (atum) and cloned into the lrg plasmid (lenti-sgrna-efs-gfp; addgene; ). next, lrg lentivirus was produced in 293t cells, concentrated using lenti-x concentrator (takara bio […]


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