CRISPR multitargeter specifications

Information


Unique identifier OMICS_08506
Name CRISPR multitargeter
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability Stable
Maintained Yes

Maintainer


  • person_outline Sergey V. Prykhozhij <>

Publication for CRISPR multitargeter

CRISPR multitargeter in publications

 (4)
PMCID: 5461336
PMID: 28638383
DOI: 10.3389/fimmu.2017.00661

[…] (px458) (addgene plasmid # 48138) was a gift from dr. feng zhang at the massachusetts institute of technology. a target-specific sgrna sequence for exon 3 of human gr gene was identified using the crispr multitargeter and the zhang lab target finder online tools. the target sgrna sequence was cloned into the cas9 vectors according to the zhang lab protocol (). in brief, two single-stranded dna […]

PMCID: 5314375
PMID: 28209983
DOI: 10.1038/srep42409

[…] were used. the sgrna guide sequence are 5′-cataatgtcatgattgatca-3′ (ck2α), 5′-gttcacctcggcgtagaccc-3′ (ck2α′), and 5′-tcctggttctgtgggctccg-3′ (ck2β). sequence for ck2β have been chosen using the crispr multitargeter tool and off targets have been excluded using gt-scan tools., c2c12 cells were cultured in six-well dishes to 70–80% confluence. cells were co-transfected with 1 μg of sgrna […]

PMCID: 4877526
PMID: 27252719
DOI: 10.3389/fpls.2016.00703

[…] http://www.rgenome.net/; casot, http://eendb.zfgenetics.org/casot/index.php; cctop, http://crispr.cos.uni-heidelberg.de/; chopchop, https://chopchop.rc.fas.harvard.edu/; cod, http://cas9.wicp.net/; crispr multitargeter, http://www.multicrispr.net/; crispr primer designer, http://www.plantsignal.cn/crispr/crispr_primer_designer.html; crisprdirect, http://crispr.dbcls.jp/; crispr-era, […]

PMCID: 4661947
PMID: 26612492
DOI: 10.1186/s13059-015-0823-x

[…] on which to prioritize among the candidate sgrnas, such as provided by the broad institute sgrna designer or other tools that employ target scoring metrics from the doench et al. study or elsewhere (crispr multitargeter [], protospacer []). for genomic regions in which traditional spcas9 pam sites might be scarce, or greater targeting specificity is required, new forms of cas9 […]


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CRISPR multitargeter institution(s)
Department of Pediatrics, Dalhousie University, Halifax, NS, Canada

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