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Protocols

CRISPR multitargeter specifications

Information


Unique identifier OMICS_08506
Name CRISPR multitargeter
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability Stable
Maintained Yes

Maintainer


  • person_outline Sergey V. Prykhozhij

Publication for CRISPR multitargeter

CRISPR multitargeter citations

 (4)
library_books

Non canonical Glucocorticoid Receptor Transactivation of gilz by Alcohol Suppresses Cell Inflammatory Response

2017
Front Immunol
PMCID: 5461336
PMID: 28638383
DOI: 10.3389/fimmu.2017.00661

[…] FP (PX458) (Addgene plasmid # 48138) was a gift from Dr. Feng Zhang at the Massachusetts Institute of Technology. A target-specific sgRNA sequence for Exon 3 of human GR gene was identified using the CRISPR MultiTargeter and the Zhang lab target finder online tools. The target sgRNA sequence was cloned into the Cas9 vectors according to the Zhang lab protocol (). In brief, two single-stranded DNA […]

library_books

Generation and quantitative proteomics analysis of CK2α/α’(−/−) cells

2017
Sci Rep
PMCID: 5314375
PMID: 28209983
DOI: 10.1038/srep42409

[…] nits were used. The sgRNA guide sequence are 5′-CATAATGTCATGATTGATCA-3′ (CK2α), 5′-GTTCACCTCGGCGTAGACCC-3′ (CK2α′), and 5′-TCCTGGTTCTGTGGGCTCCG-3′ (CK2β). Sequence for CK2β have been chosen using the CRISPR MultiTargeter tool and off targets have been excluded using GT-Scan tools.C2C12 cells were cultured in six-well dishes to 70–80% confluence. Cells were co-transfected with 1 μg of sgRNA plasmid […]

library_books

CRISPR/Cas9 in zebrafish: an efficient combination for human genetic diseases modeling

2016
Hum Genet
PMCID: 5214880
PMID: 27807677
DOI: 10.1007/s00439-016-1739-6

[…] designed for CRISPR/Cas9 system in zebrafish. Most of them are websites or softwares designed to assemble sgRNAs with minimized off-target effects based on the wild type genomic sequences, including CRISPR MultiTargeter, CRISPRdirect, CCTop, CHOPCHOP, sgRNAcas9, CRISPRscan and so on (Xie et al. ; Montague et al. ; Prykhozhij et al. ; Stemmer et al. ; Naito et al. ; Moreno-Mateos et al. ). In addi […]

call_split

Resources for the design of CRISPR gene editing experiments

2015
Genome Biol
PMCID: 4661947
PMID: 26612492
DOI: 10.1186/s13059-015-0823-x
call_split See protocol

[…] on which to prioritize among the candidate sgRNAs, such as provided by the Broad Institute sgRNA Designer or other tools that employ target scoring metrics from the Doench et al. study or elsewhere (CRISPR MultiTargeter [], Protospacer []). For genomic regions in which traditional SpCas9 PAM sites might be scarce, or greater targeting specificity is required, new forms of Cas9 have been leveraged […]

Citations

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CRISPR multitargeter institution(s)
Department of Pediatrics, Dalhousie University, Halifax, NS, Canada

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