Serves for optimal design of sgRNAs. CRISPys is an algorithm that can be used for the conception of clustered regularly interspaced short palindromic repeats (CRISPR) systems given a set of redundant genes. It harnesses the lack of specificity of the CRISPR-CRISPR associated protein 9 (Cas9) genome editing technique, providing researchers the ability to simultaneously mutate multiple genes. It detects highly similar sequences among the set of all potential CRISPR-Cas9 targets located within the genes of interest, and then designs sgRNAs.
School of Plant Sciences and Food security, Tel Aviv University, Ramat Aviv, Israel; Department of Computer Science, University of California, Los Angeles, CA, USA
CRISPys funding source(s)
Supported by a grant from the Chief Scientist of the Israeli Ministry of Agriculture and Rural Development (1832/14), a grant from the European Research Council Starting Grant (757683-RobustHormoneTrans), MSc fellowships provided by the Manna Program in Food Safety and Security and the Edmond J. Safra Center for Bioinformatics at Tel-Aviv University and PhD fellowship provided by the Rothschild Caesarea Foundation.