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Facilitates the application of BLAST+ in the analysis of Next Generation Sequencing (NGS) data. CrocoBLAST runs a pre-calculation to estimate the optimal size of the temporary input files so that maximum speed-up can be achieved on a given machine without negative effect on memory usage. It detects the maximum number of threads for the machine. The tool improves the user experience when working with large data. It is useful for NGS applications that involve annotation as an essential task.

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CrocoBLAST versioning

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CrocoBLAST classification

CrocoBLAST specifications

Software type:
Restrictions to use:
Programming languages:
C, Java
Graphical user interface
Operating system:
Unix/Linux, Mac OS, Windows
Computer skills:

CrocoBLAST support


  • Jaroslav Koca <>
  • CrocoBLAST Team <>

Additional information

http://webchem.ncbr.muni.cz/Wiki/CrocoBLAST:UserManual http://webchem.ncbr.muni.cz/Platform/App/CrocoBLAST#documentation


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CEITEC-Central European Institute of Technology, Faculty of Science, Masaryk University, Brno, Czech Republic; National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic; Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

Funding source(s)

Supported by the Ministry of Education, Youth and Sports of the Czech Republic (project CEITEC 2020: LQ1601) and the National Counsel of Technological and Scientific Development of Brazil (305833/2013-8).

Link to literature

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