CTF estimation/correction software tools | Cryo-EM analysis

Contrast transfert function estimation/correction software tools | Cryo-electron microscopy image analysis

Cryo-EM images are usually collected in defocused condition for increased phase contrast. These images are modulated in frequency space by the phase CTF (Contrast Transfer Function). Image CTF must be estimated for later deconvolution. Currently, several software for CTF estimation is available (e.g. ctffind, gctf, e2ctf.py).

SPIDER / System for Processing Image Data from Electron microscopy and Related fields

Describes the reconstruction of biological molecules from the electron micrographs of single particles. SPIDER is an open source project that manages computation using the image-processing software and can also using a graphical user interface (GUI), termed the SPIDER Reconstruction Engine. These two approaches are described to obtain an initial reconstruction: random-conical tilt and common lines.

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