CTFFIND specifications


Unique identifier OMICS_15823
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Programming languages C++, Fortran
License Other
Computer skills Advanced
Version 4.1
Stability Stable
Maintained Yes


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  • person_outline Nikolaus Grigorieff <>

CTFFIND articles

CTFFIND citations

PMCID: 5754084

[…] with 11 boxable virions is displayed in fig 1. averaged power spectra for the micrographs was generated in spider [27]. the contrast transfer function (ctf) parameters were calculated with ctffind3 [28]. boxed particles were preprocessed for normalization and linear gradient using the robem function in the auto3dem software package [29]. the single particle reconstruction was computed […]

PMCID: 5018250

[…] size of 1.1å/pixel. images were taken within a defocus range of −0.5 to −3.5 μm., the spider software package (frank et al., 1996) was used for most operations, unless otherwise noted. the program ctffind3 (mindell and grigorieff, 2003) was used for determining defocus values. for the ped208 filaments, 362 images were used after removing those with drift, a poor contrast transfer function […]

PMCID: 4785225

[…] into segments of size 512 × 512 pixels with a shift of two subunits along the helical axis by the eman boxer program (ref. 54). the defocus value of each micrograph was evaluated using the program ctffind3 (ref. 55). an initial density map was reconstructed from binned segments of 256 × 256 pixels using iterative helical real-space reconstruction29. the refined helical parameters corresponded […]

PMCID: 4453056

[…] data were collected on a tecnai g2 polara tem (fei) operated at 300 kv, and micrographs were scanned on a heidelberg drum scanner with a pixel size of 1.23 å on the object scale. ctf correction by ctffind (mindell and grigorieff 2003), automated particle picking by signature (chen and grigorieff 2007), and image processing by spider (frank et al. 1996) were performed essentially as described […]

PMCID: 4416312

[…] based helical reconstruction package that was originally inspired by the ihrsr approach (egelman, 2000). briefly, the ctf parameters were determined using micctfdetermine that relies on ctffind with subsequent ctftilt measurements (mindell and grigorieff, 2003). we used the diagnostic output of micctfdetermine to discard micrographs that did not show thon rings exceeding 6 å. 80% […]

CTFFIND institution(s)
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA

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