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Ctrax specifications


Unique identifier OMICS_12999
Name Ctrax
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Input data Video
Input format Fly Movie Format (FMF), Static Background Fly Movie Format (SBFMF)
Output data Trajectories, tracking data
Output format MAT, CSV
Operating system Unix/Linux, Mac OS, Windows
Programming languages Python
License GNU General Public License version 3.0
Computer skills Medium
Version 0.5.8
Stability Stable
Maintained Yes


  • Invertebrates
    • Drosophila melanogaster



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  • person_outline Kristin Branson <>

Publication for Ctrax

Ctrax in publications

PMCID: 5897729
PMID: 29590638
DOI: 10.1242/dmm.032482

[…] video acquisition was started. locomotion was recorded using a logitec webcam and the handyavi program. the time lapse was set to 10 images/s, and 7 min were recorded. tracking was performed with ctrax and fixerrors matlab gui using the guidelines of . for the climbing assay, 10 flies were placed in a vial of 23 mm diameter and 18.5 cm height, and allowed to acclimate for 5 min. the time […]

PMCID: 5798363
PMID: 29339481
DOI: 10.1073/pnas.1716612115

[…] edmund optics, inc.). for each trial, a single fly was filmed for 12 min starting within 2 s after its introduction to the arena. the fly’s translational movements (fig. s6c) were determined using ctrax (); the various measures and classification of behaviors, including the exact coordinates of the post for each trial, were generated using custom software written in matlab (mathworks)., flies […]

PMCID: 5790380
PMID: 29345616
DOI: 10.7554/eLife.32018.030

[…] and the bonferroni multiple comparisons posthoc test using prism graphpad 6., in some experiments flies were videotracked by using an infrared camera on the top of the climate box and analyzed with ctrax software., 200 fly embryos (12 hr ael) were collected in a petri dish, washed with 10% bleach once, in 70% ethanol twice, in pbs once, and in sterile, distilled water twice. these embryos […]

PMCID: 5633173
PMID: 29016648
DOI: 10.1371/journal.pone.0185968

[…] activity measurement is similar to that of cole [] in the study on the activity behavior of the ant temnothorax albipennis., the x-y coordinates of the individual positions were extracted using the ctrax software (caltech multiple fly tracker; version 0.3.12) and the associated fixerrors toolbox for matlab (v. 7.10.0 2010; mathworks, inc., natick, ma, usa [–]; matlab v. 7.10.0). ctrax […]

PMCID: 5577484
PMID: 28855361
DOI: 10.1098/rspb.2017.1126

[…] of shoals of two fish (n = 115 male trials, n = 109 female trials) and ∼35 h of footage of shoals of eight fish (n = 51 male trials, n = 78 female trials)., we tracked the pairs of fish using ctrax [] semi-automated tracking software and corrected any errors the software made using the fixerrors gui in matlab. we tracked the groups of eight fish using didson tracking software [] […]

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Ctrax institution(s)
California Institute of Technology, Pasadena, CA, USA; Case Western Reserve University, Cleveland, OH, USA
Ctrax funding source(s)
Funding for this research was provided by National Institutes of Health grant R01 DA022777.

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