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Protocols

CytoD specifications

Information


Unique identifier OMICS_03004
Name CytoD
Alternative name Cytogenetics Database
Restrictions to use None
Community driven No
Data access File download, Browse
User data submission Not allowed
Version 1.0
Maintained Yes

Taxon


  • Primates
    • Homo sapiens

Maintainer


  • person_outline Madan Babu

CytoD citations

 (7)
library_books

Differential Phagocytic Properties of CD45low Microglia and CD45high Brain Mononuclear Phagocytes—Activation and Age Related Effects

2018
Front Immunol
PMCID: 5840283
PMID: 29552013
DOI: 10.3389/fimmu.2018.00405

[…] or microsphere phagocytosis studies, distinct peaks were identified in the cell distribution while for Aβ42 phagocytosis, a shift in fluorescence and a second peak of fluorescent cells were observed. CytoD pre-incubation experiments allowed us to confirm actin-dependence of these observed responses. For cell sorting, washed BV2 cells were subjected to FACS using FACSAria IIB instrument as per stan […]

library_books

Neurite outgrowth is driven by actin polymerization even in the presence of actin polymerization inhibitors

2016
Mol Biol Cell
PMCID: 5170553
PMID: 27682586
DOI: 10.1091/mbc.E16-04-0253

[…] cs (), neurons were nucleofected at the time of plating with F-tractin–tdTomato using Neon Transfection system (Invitrogen), cultured for 48 h in glass-bottomed dishes, treated with 1 μM LatB or 1 μM CytoD for 24 h, and imaged by fluorescence and phase contrast microscopy for 15 min at the rate of 1 frame/min in an environmental chamber maintained at 37°C in humidified atmosphere with 5% CO2. For […]

call_split

Effect of IVIG Formulation on IgG Binding to Self and Exo Antigens In Vitro and In Vivo

2016
PLoS One
PMCID: 4999199
PMID: 27561008
DOI: 10.1371/journal.pone.0161826
call_split See protocol

[…] cchini, University of Perugia (Perugia, Italy) []) according to Webster et al. [] with some modifications []. In addition to Privigen Fc fragment, which does not mediate phagocytosis, Cytochalasin D (CytoD), which blocks receptor-mediated endocytosis, was used as control. Propidium iodide staining was performed to determine the number of dead cells. After incubation the cells were subjected to FAC […]

library_books

Multi walled carbon nanotube induces nitrative DNA damage in human lung epithelial cells via HMGB1 RAGE interaction and Toll like receptor 9 activation

2016
PMCID: 4812657
PMID: 27026438
DOI: 10.1186/s12989-016-0127-7

[…] O synthase (iNOS) and endocytosis in MWCNT-induced 8-nitroG formation, 1 μM 1400 W, 10 μM Bay11-7082 (Bay), 2 mM methyl-β-cyclodextrin (MBCD), 50 μM monodansylcadaverine (MDC) or 1 μM cytochalasin D (CytoD) was added before MWCNT treatment. These inhibitors were purchased from Sigma-Aldrich (St. Louis, MO, USA). We employed these concentrations of the inhibitors, because they did not show signific […]

library_books

The Rhoptry Pseudokinase ROP54 Modulates Toxoplasma gondii Virulence and Host GBP2 Loading

2016
PMCID: 4863586
PMID: 27303719
DOI: 10.1128/mSphere.00045-16

[…] y seen with other ROP effectors (, ). To evaluate whether ROP54 is an injected effector, we carried out “evacuole” assays, in which parasites are unable to invade due to inhibition by cytochalasin D (CytoD) treatment but still able to release streams of rhoptry proteins into the cytosol of the host cell (, ). Using ROP54HAII parasites, we were able to observe classic “strings” of HA-positive evacu […]

library_books

Dynamin 2 Regulates Fusion Pore Expansion and Quantal Release through a Mechanism that Involves Actin Dynamics in Neuroendocrine Chromaffin Cells

2013
PLoS One
PMCID: 3734226
PMID: 23940613
DOI: 10.1371/journal.pone.0070638

[…] Cultured chromaffin cells were incubated for 10 minutes at 37°C with 4 µM CytoD or for 1 hour with 100 µM dynasore, fixed for 1 h with 2.5% glutaraldehyde and 2% paraformaldehyde in 0.1 M sodium cacodylate and then rinsed and post fixed for 2 h with 1% osmium tetroxide and […]


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CytoD institution(s)
MRC Laboratory of Molecular Biology, Cambridge, UK

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