Computational protocol: The diagnostic value of matrix metalloproteinase 9 (MMP-9) and tissue inhibitor of matrix metalloproteinases 1 (TIMP-1) determination in the sera of colorectal adenoma and cancer patients

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Protocol publication

[…] A preliminary statistical analysis (χ2 test) revealed that serum levels of MMP-9, TIMP-1, CEA, and CA 19-9 did not follow a normal distribution. Therefore, nonparametric statistical analyses were used. The stages B1 and B2 were analyzed as one group (stage B), whereas stages C1 and C2—as stage C because of small numbers of patients in the particular subgroups. The Mann–Whitney U test was used to compare the two groups in each category (the controls versus CRC or CA patients; M0 versus M1 group; the group of patients who survived versus patients who died of CRC; resectable tumors versus nonresectable). Differences between more than two groups (e.g., stage B, C, D; or T2, T3, T4; or N0, N1, N2, N3) were compared using ANOVA on ranks (Kruskal–Wallis tests). If significant differences were found, the post hoc Dwass–Steele–Critchlow–Fligner test was conducted to determine which groups were different. Differences were considered statically significant with p values below 0.05. Moreover, we calculated diagnostic criteria such as percentage of elevated levels (diagnostic sensitivity) and area under the ROC curve (AUC) for the MMP-9, TIMP-1, and tumor markers. Statistical analyses were carried out using the STATISTICA 5.1 PL program (StatSoft Inc., Tulsa, OK). Diagnostic criteria and the ROC curves were calculated using MedCalc statistical software (MedCalc Software, Mariakerke, Belgium) and Microsoft Office Excel program (Microsoft Corporation, Redmond, WA). […]

Pipeline specifications

Software tools Statistica, MedCalc
Application Miscellaneous
Organisms Homo sapiens
Diseases Neoplasms, Colorectal Neoplasms