Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 18
Release date: Aug 15 2007
Last update date: Apr 5 2017
Access: Public
Taxon: Homo sapiens
Dataset link Profiling Motility Signal-Induced Genes in Human Keratinocytes

Experimental Protocol


HKs were seeded on collagen I pre-coated tissue culture plates (150 mm) around 45% confluence (~ 2.5 X 106 cells) in complete medium and incubated overnight. Next day, the cells (~ 4.5 X 106 cells) were deprived of any supplemental GFs and incubated in serum-free medium for 16 hours to rest the cells at G0/G1 phase. The cells were then washed with pre-warmed serum-free medium and incubated in fresh serum-free medium containing 20 ng/ml TGFbeta for 20 min at 37oC to block expression of growth signal-related genes. TGFalpha or insulin was added to separate plates (in the continued presence of TGFbeta) for 30 min, 60 min and 120 min. At the end of each stimulation time points, the cells were washed with ice-cold PBS to holt the stimulation and subjected to RNA isolation.

Repositories


GEO

GSE8531

ArrayExpress

E-GEOD-8531

BioProject

PRJNA101663

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Contact


CHIEH-FANG CHENG