Computational protocol: Functional analysis of GbAGL1, a D-lineage gene from cotton (Gossypium barbadense)

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Protocol publication

[…] The amino acid sequences of the proteins used in this study were obtained from the GenBank database. Multiple sequence alignment was performed using ClustalX 1.83 (http://www.ebi.ac.uk). The phylogenetic tree was constructed using Molecular Evolutionary Genetics Analysis (MEGA) software 3.1 (). The Neighbor–Joining tree method was adopted and the tree nodes were evaluated by bootstrap analysis for 1000 replicates. The isoelectric point (pI) and molecular weight (MW) were predicted online (http://www.swiss-prot.com). The 20 MADS proteins used in this study, and their accession numbers, are listed in . […]

Pipeline specifications

Software tools Clustal W, MEGA
Application Phylogenetics
Organisms Gossypium hirsutum, Gossypium barbadense, Arabidopsis thaliana