Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 2
Release date: Jul 17 2016
Last update date: Aug 10 2018
Access: Public
Diseases: Colorectal Neoplasms
Genes: EGFR
Dataset link Global gene expression analysis between cetuximab-sensitive and cetuximab-resistant colorectal cancer cells

Experimental Protocol


We generated a cetuximab-resistant DiFi subline, termed DiFi5, by chronic exposure of parental DiFi cells to serially increased doses of cetuximab (from 0.5 nM to 5 nM) for over 1 year. The resulting DiFi5 subline exhibits significant resistance to cetuximab-induced apoptosis. After we confirmed that the phenotype was stable over a period of time of more than 6 months, the RNA from DiFi and DiFi5 cells were extracted and hybridized to an Affymetrix HG-U133A array according to the manufacturer’s instructions. Following the hybridization, the array was scanned using a laser confocal scanner, and microarray image data were analyzed using DNA-Chip Analyzer (dChip), version 1.3, by the Sequencing and Microarray Facility at MD Anderson Cancer Center.

Repositories


GEO

GSE71210

ArrayExpress

E-GEOD-71210

BioProject

PRJNA290584

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Contact


Zhen Fan