Dataset features


Application: RNA-seq analysis
Number of samples: 49
Release date: Oct 16 2009
Last update date: Aug 1 2018
Access: Public
Taxon: Drosophila melanogaster
Dataset link Discrete genomic domains exhibit co-occurrence of H3K9me3 and H3K27me3 histone modifications throughout Drosophila

Experimental Protocol

In this study we have performed ChIP-chip in triplicate for 2 repressive histone marks in Drosophila: H3K27me3 and H3K9me3. Hybridizations on Agilent microarrays have been perfomed to compare with Input chromatin sample as a negative control to calculate enrichments. ChIP-chip experiments have been performed for 9 developmental time points: E0-4h; E4-8h; E12-16h; E16-20h; E20-24h; L1; L2; Pupae and Adult Males. In addition, 3 ChIP-seq experiments have been performed for validation for E-0-4h, L1 and Pupae time-points where one replicate has been analyzed on the Solexa platform. To complement this study, total RNA (Poly-A +) has been sequenced on the Solexa platform (2 lanes each) for matching time-points, in order to survey the gene expression levels associated with the ChIP experiments.






Kevin White