Expression data from Arabidopsis seed compartments at the bending coyledon stage

We collected bending-cotyledon seed compartments from 7 micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments from seeds containing bending cotyledon stage embryos. For the purposes of this study we captured 6 compartments: embryo proper, mycropylar endosperm, cellularized peripherial endosperm, chalazal endosperm, chalazal seed coat and seed coat, in addition to serial sections encompasing the entire bending-cotyledon stage seed.

Dataset features

Information

Specifications

Application:	Gene expression microarray analysis
Number of samples:	14
Release date:	Jan 27 2010
Last update date:	Aug 15 2018
Access:	Public
Dataset link	Expression data from Arabidopsis seed compartments at the bending coyledon stage

Experimental Protocol

Bending-cotyledon stage seed compartments were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 14 samples (embryo proper, micropylar endosperm, peripherial endosperm, chalazal endosperm, chalazal seed coat, seed coat and whole seeds, 2 biological replicates each).

Repositories

GEO

GSE20039

ArrayExpress

E-GEOD-20039

BioProject

PRJNA124299

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Contact

John Harada

Ryan Christopher Kirkbride