Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 136
Release date: May 26 2016
Last update date: Nov 20 2018
Access: Public
Taxon: Danio rerio
Dataset link Whole organism lineage tracing by combinatorial and cumulative genome editing

Experimental Protocol


The GESTALT lineage tracing barcode was introduced into human cell culture (HEK293T) or the Danio rerio model organism. Mutations in this barcode, introduced by the CRISPR/Cas9 system, persist through cell divisions, and provide a means to trace the shared lineage of cells. These compact barcodes were read out by sequencing either the RNA or DNA of specific cell populations. Specifics of the protocol can found in the accompanying citation.The GESTALT lineage tracing barcode was introduced into human cell culture (HEK293T) or the Danio rerio model organism. Mutations in this barcode, introduced by the CRISPR/Cas9 system, persist through cell divisions, and provide a means to trace the shared lineage of cells. These compact barcodes were read out by sequencing either the RNA or DNA of specific cell populations. Specifics of the protocol can found in the accompanying citation.

Repositories


GEO

GSE81713

ENA

SRP075493

BioProject

PRJNA322340

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