Computational protocol: Plasmid transferability of KPC into a virulent K2 serotype Klebsiella pneumoniae

Similar protocols

Protocol publication

[…] In vitro and in vivo assays to assess virulence were performed by serum resistance, neutrophil phagocytosis and mice lethality experiments. Neutrophil phagocytosis was performed as previously described []. The recipient serotype K2 K. pneumoniae (NJ-K2) isolated from a liver abscess was used as a control strain []. This strain is hypervirulent with a median lethal dose (LD50) < 102 colony forming unit (CFU) and is highly phagocytic resistance (< 10% of phagocytic rate 15 minutes).For the determination of LD50 in mice, pathogen-free, adult, 6-8-week-old, male BALB/c mice weighing 20–25 g were obtained from the National Laboratory Animal Center. This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All animal care procedures and protocols were approved by the Institutional Animal Care and Use Committee of the National Health Research Institute (NHRI-IACUC-096004-A). Tenfold serial dilutions of KP were made from a starting concentration of 106 CFU, and the adult BALB/c mice had intraperitoneal injections with 0.1 ml of each concentration. Six mice were used as a sample population for each bacterial concentration []. Signs and symptoms of infection were observed for 14 days.Survival of the inoculated mice was recorded, and the LD50 was calculated using SigmaPlot version 7.0 from SPSS Inc. […]

Pipeline specifications

Software tools SigmaPlot, SPSS
Application Miscellaneous
Organisms Klebsiella pneumoniae, Mus musculus
Diseases Klebsiella Infections
Chemicals Ampicillin, beta-Lactams