Dataset features

Specifications


Application: ChIP-on-chip analysis
Number of samples: 7
Release date: Mar 7 2013
Last update date: May 27 2014
Access: Public
Dataset link Histone H3 lysine9 acetylation, rather than lysine4 trimethylation, marks meiotic recombination hotspots and promotes recombination initiation in fission yeast

Experimental Protocol


S.pombe cells in a pat1-114 background were induced to enter meiosis by the haploid meiosis system, and harvested one hour after the induction. ChIP were performed using anti-H3Cter, H3K9ac, -H3K14ac and -H3K4me3 antibodies. pat1-114 rad50S rec12+-FLAG cells in a wild type, H3K9A or set1+ deletion background were induced to enter meiosis by the haploid meiosis system, and harvested five hours after the induction. ChIP were performed using anti-FLAG antibodies.

Repositories


GEO

GSE31648

ArrayExpress

E-GEOD-31648

BioProject

PRJNA145419

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Contact


Shintaro YAMADA

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