Dataset features

Specifications


Dataset type: Expression profiling by high throughput sequencing
Number of samples: 12
Release date: Feb 4 2019
Last update date: Feb 6 2019
Access: Public
Dataset link Identification of Novel Regulatory Genes in APAP Induced Hepatocyte Toxicity by a Genome-Wide CRISPR-Cas9 Screen

Experimental Protocol


Single-read DNA amplicon sequencing of a CRISPR/Cas9 genome-wide knockout screen of acetaminophen-induced hepatotoxicity in the HuH7 human hepatocarcinoma cell line. sgRNA library transfer plasmid isolated from E. coli was sequenced alongside the screen to assess library representation. For each sample there are 4 technical replicates.

Repositories


GEO

GSE112463

ENA

SRP136733

BioProject

PRJNA447595

Download