Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 2
Release date: Mar 31 2009
Last update date: Aug 23 2018
Access: Public
Diseases: Deficiency Diseases
Chemicals: Androgens
Dataset link Alterations in Cell Growth and Signaling in ErbB3 Binding Protein -1 (Ebp1) Deficient Mice

Experimental Protocol


Isolation of Mouse Embryo Fibroblasts (MEFS) and proliferation assays. Embryos (12.5 days) were minced and incubated in 0.25% trypsin and 5 U of DNAse I for 37º C for 30 min. Dulbecco’s MEM supplemented with 10% fetal bovine serum and penicillin and streptomycin were added to the cell suspension and cells centrifuged at 1000 rpm for 10 min. The pellet was resuspended in medium and cells were grown in MEM and passaged by trypsinization.

Repositories


GEO

GSE13718

ArrayExpress

E-GEOD-13718

BioProject

PRJNA110395

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Contact


Anne Hamburger

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