|Application:||Gene expression microarray analysis|
|Number of samples:||22|
|Release date:||Apr 7 2008|
|Last update date:||Jul 8 2016|
|Taxon:||Homo sapiens, Mus musculus|
|Dataset link||Expression Profiling of the Developing Mouse Lung|
RNA was isolated from individual (E18, P1, P4, P7, P10, P14, P21, and adult) or pooled (E12, E14, E16) whole lungs using a modified guanidinium:phenol extraction method.Ten micrograms of total RNA was used to generate target cRNA for hybridization to Affymetrix Mu11K chipset subA and subB oligonucleotide microarrays. A single chip set was used for each time-point. Before target generation, individual RNA samples were pooled so that each target was derived from a minimum of three individual lungs.