Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 22
Release date: Apr 7 2008
Last update date: Jul 8 2016
Access: Public
Taxon: Homo sapiens, Mus musculus
Dataset link Expression Profiling of the Developing Mouse Lung

Experimental Protocol


RNA was isolated from individual (E18, P1, P4, P7, P10, P14, P21, and adult) or pooled (E12, E14, E16) whole lungs using a modified guanidinium:phenol extraction method.Ten micrograms of total RNA was used to generate target cRNA for hybridization to Affymetrix Mu11K chipset subA and subB oligonucleotide microarrays. A single chip set was used for each time-point. Before target generation, individual RNA samples were pooled so that each target was derived from a minimum of three individual lungs.

Repositories


GEO

GSE10889

ArrayExpress

E-GEOD-10889

BioProject

PRJNA107287

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Contact


Soumyaroop Bhattacharya