Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 54
Release date: Dec 17 2012
Last update date: Aug 13 2018
Access: Public
Diseases: Down Syndrome, Glioma, Neural Tube Defects
Dataset link Integration-Free Induced Pluripotent Stem Cells Model Genetic and Neural Developmental Features of Down Syndrome Etiology

Experimental Protocol


Control (WT) and Down Syndrome iPSC lines were generated via episomal reprogramming of human donor fibroblasts. Two iPSC clones conforming to iPS criteria (determined by immunocytochemistry detection of pluripotency markers) were developed from each fibroblast donor. Two control (WT) and DS lines each were further characterized and underwent neural differentiation. Multiple biological replicates of donor fibroblast and iPSC from both control (WT) and DS lines, including 3 euploid DS samples and 3 MEL1 hESC controls (total 54 samples) were hybridized to Illumina HT-12 v4 microarray for gene expression analysis.

Repositories


GEO

GSE42956

ArrayExpress

E-GEOD-42956

BioProject

PRJNA183939

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