Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 33
Release date: Nov 1 2011
Last update date: Oct 29 2018
Access: Public
Diseases: Breast Neoplasms, Neoplasms
Chemicals: Pentosephosphates
Dataset link 18F-fluorodeoxy-glucose positron emission tomography marks MYC-overexpressing human basal-like breast cancers.

Experimental Protocol


Our general strategy to identify molecular determinants of FDG-retention through a genome-wide approach consisted of FDG uptake measurements in cancer cell lines and primary human tumors, the selection of samples with particularly high versus low FDG-retention, and subsequent pathway-based analysis of RNA expression data collected from these samples. Cell line RNA was extracted from a 10 cm plate seeded simultaneously and at the same density as the wells for FDG-uptake assays. For primary human tumor samples, RNA was extracted from macrodissected frozen tumor tissue. All cell line RNA samples and the astrocytoma RNA aliquots were hybridized to Affymetrix U133 Plus 2.0 arrays. All primary breast cancer RNA samples were hybridized to Affymetrix U133A arrays.

Repositories


GEO

GSE21217

ArrayExpress

E-GEOD-21217

BioProject

PRJNA126315

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