|Application:||Gene expression microarray analysis|
|Number of samples:||33|
|Release date:||Nov 1 2011|
|Last update date:||Oct 29 2018|
|Diseases:||Breast Neoplasms, Neoplasms|
|Dataset link||18F-fluorodeoxy-glucose positron emission tomography marks MYC-overexpressing human basal-like breast cancers.|
Our general strategy to identify molecular determinants of FDG-retention through a genome-wide approach consisted of FDG uptake measurements in cancer cell lines and primary human tumors, the selection of samples with particularly high versus low FDG-retention, and subsequent pathway-based analysis of RNA expression data collected from these samples. Cell line RNA was extracted from a 10 cm plate seeded simultaneously and at the same density as the wells for FDG-uptake assays. For primary human tumor samples, RNA was extracted from macrodissected frozen tumor tissue. All cell line RNA samples and the astrocytoma RNA aliquots were hybridized to Affymetrix U133 Plus 2.0 arrays. All primary breast cancer RNA samples were hybridized to Affymetrix U133A arrays.