Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 9
Release date: Jul 1 2010
Last update date: Oct 29 2018
Access: Public
Diseases: Epilepsy, Neuroblastoma
Chemicals: Phenytoin, Tachykinins
Genes: REST, BDNF
Dataset link Impact of NRSF variant over-expression in SK-N-AS human neuroblastoma cells

Experimental Protocol


Human SK-N-AS cells were treated with expression constructs over-expressing either the full length human transcription factor, NRSF, (via the RE-EX1 construct) or a truncated variant (via the HZ4 construct). Cells were treated for either 0hrs (base line controls), 24hrs or 48hrs, before being immediately processed for RNA extraction. An affymetrix microarray was employed to investigate gene expression patterns, comparing each time point (24hrs or 48hrs) againsts its baseline ()hrs) control. This experiment was performed in triplicate samples per batch, and over three batches.

Repositories


GEO

GSE22467

ArrayExpress

E-GEOD-22467

BioProject

PRJNA128615

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Contact


Stuart Gillies