Computational protocol: Essential requirement for two-pore channel 1 in NAADP-mediated calcium signaling

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Protocol publication

[…] BlastP and TBlastN searches () were performed against the genomic and protein databases of the National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov/blast/) and the Department of Energy Joint Genome Institute (http://genome.jgi-psf.org/) using protein sequences of human and sea urchin (Stronglyocentrotus purpuratus) TPCs. Sequence datasets were retrieved for sequence alignment followed by manual editing and phylogenetic analysis, essentially as described previously (; ). […]

Pipeline specifications

Software tools BLASTP, TBLASTN
Applications Phylogenetics, Amino acid sequence alignment
Organisms Homo sapiens
Chemicals Calcium