|Application:||Gene expression microarray analysis|
|Number of samples:||24|
|Release date:||Dec 31 2009|
|Last update date:||Oct 29 2018|
|Computational protocol:||GC–RMA, GeneSpring GX|
|Dataset link||Fine-tuning of human dendritic cells regulation revealed by translational profiling|
To identify translationally regulated mRNA molecules, gene expression derived from the polysome-bound mRNAs was compared by Affymetrix microarrays analysis to the gene expression derived from unfractionated total mRNAs derived from whole-cell lysates, as recently described on several reports (Johannes 1999, Rajasekhar 2003, Bushell 2006, Lü 2006, Parent 2008). Polysomal RNA (P) and total RNA (T) were isolated from MoDCs generated from four different blood donors. Since three timepoints (0h, 4h and 16h) were chosen for each blood donor and RNA type, twenty-four RNA samples were totally analyzed by microarrays.
DOI: 10.1186/1745-7580-5-5call_split See protocol