Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 24
Release date: Dec 31 2009
Last update date: Oct 29 2018
Access: Public
Taxon: Homo sapiens
Computational protocol: GC–RMA, GeneSpring GX
Dataset link Fine-tuning of human dendritic cells regulation revealed by translational profiling

Experimental Protocol


To identify translationally regulated mRNA molecules, gene expression derived from the polysome-bound mRNAs was compared by Affymetrix microarrays analysis to the gene expression derived from unfractionated total mRNAs derived from whole-cell lysates, as recently described on several reports (Johannes 1999, Rajasekhar 2003, Bushell 2006, Lü 2006, Parent 2008). Polysomal RNA (P) and total RNA (T) were isolated from MoDCs generated from four different blood donors. Since three timepoints (0h, 4h and 16h) were chosen for each blood donor and RNA type, twenty-four RNA samples were totally analyzed by microarrays.

Repositories


GEO

GSE14000

ArrayExpress

E-GEOD-14000

BioProject

PRJNA112501

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Contact


Philippe Pierre

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