Dataset features

Specifications


Application: Gene expression microarray analysis, miRNA array analysis
Number of samples: 8
Release date: Jun 12 2015
Last update date: May 2 2017
Access: Public
Diseases: Hematologic Diseases, Multiple Myeloma, Neoplasms
Dataset link Inactivation of the exosome ribonuclease DIS3 triggers the pluripotency factor LIN28B, repressing let-7 miRNAs and unleashing MYC and RAS.

Experimental Protocol


This series of microarray experiments contains the miRNA expression profiles of independent replicates of RPMI-8226, KMS12-BM multiple myeloma cell lines and HEK-293T cells, knocked-down with a scrambled or hDIS3 sh4 and collected 72 hours after infection. 500 nanograms of total RNA were processed using the FlashTag labeling kit, which uses a tailing reaction followed by ligation of the biotinylated signal molecule to the target RNA sample. The labelled RNA was then hybridized to Affymetrix GeneChip® microRNA arrays v1.0, following the Affymetrix manufacturer's instructions.

Repositories


GEO

GSE55246

ArrayExpress

E-GEOD-55246

BioProject

PRJNA238992

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Contact


Luca Agnelli
Antonino Neri

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