Dataset features

Specifications


Application: Gene expression microarray analysis, miRNA array analysis
Number of samples: 12
Release date: Mar 31 2014
Last update date: Jun 30 2014
Access: Public
Taxon: Sus scrofa
Computational protocol: Microprocessor SVM, TargetScan, miRBase
Dataset link Identification of the 3' end of the pig miRNA by the RAKE technology [platform 2: 9003179]

Experimental Protocol


90K microarray was hybridized with a pool of small RNAs from 20 different tissues (superior vena cava, adipose tissue, lung, spleen, stomach, liver, intestine, kidney, descending aorta, left atrium, left ventricle, skeletal muscle, pulmonary aorta, skin, tongue, ascending aorta, arterial white cells blood, venal white cells blood, coronary valve, lymph node). Same experiment was replicated four times to grant the reliability of the identified 3’ end of miRNAs and microarray slides were scanned three times at low, medium and high PMT to improve microarray dynamic range.

Repositories


GEO

GSE28138

ArrayExpress

E-GEOD-28138

BioProject

PRJNA143153

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Contact


Gerolamo Lanfranchi

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