Computational protocol: The LetA/S two-component system regulates transcriptomic changes that are essential for the culturability of Legionella pneumophila in water

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Protocol publication

[…] NCBI Conserved Domain Database (CDD https://www.ncbi.nlm.nih.gov/cdd/) was used to search for conserved protein domains that may be implicated in signal sensing within LetS. The Accession number YP_095929.1 representing Lpg1912 of Legionella pneumophila Philadelphia-1 strain was queried. The TMHMM server v. 2.0 (http://www.cbs.dtu.dk/services/TMHMM/) and TOPCONS (http://topcons.net/) were used to predict the transmembrane helices on the N-terminal of LetS,. […]

Pipeline specifications

Software tools TMHMM, TOPCONS
Databases CDD
Application Membrane protein analysis
Organisms Legionella pneumophila