Computational protocol: Comparative Structural Analysis of Human DEAD-Box RNA Helicases

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Protocol publication

[…] All proteins were expressed in Escherichia coli as N-terminally hexahistidine tagged fusion proteins, and purified by nickel affinity chromatography and gel filtration. Proteins were crystallized in sitting drops at 4°C or 20°C. X-ray diffraction data were collected at the APS (Chicago, USA), the BESSY (Berlin, Germany), the Diamond (Oxfordshire, UK), the ESRF (Grenoble, France), and the MaxLab (Lund, Sweden) synchrotron radiation facilities. Data were indexed and integrated using XDS , MOSFLM , or DENZO , and scaled using XSCALE , SCALA or SCALEPACK . Structures were solved by molecular replacement using PHASER or MOLREP , and refined using REFMAC . Refinement rounds were complemented with manual rebuilding using COOT .Detailed can be found in . […]

Pipeline specifications

Software tools XDS, iMosflm, CCP4, Molrep, Coot
Applications Small-angle scattering, Protein structure analysis
Organisms Homo sapiens
Diseases Neoplasms
Chemicals Adenosine Triphosphate, Hydrogen