Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 18
Release date: Sep 23 2017
Last update date: Nov 30 2018
Access: Public
Chemicals: Nucleotides, Thiouridine
Dataset link Thiol-linked alkylation for the metabolic sequencing of RNA [SLAM-seq in wildtype and Xpo5 knockout mES cells]

Experimental Protocol


Wildtype (wt) mouse embryonic stem (mES) cells, clonal mES cells that had been transfected with non-targeting control guide RNAs (ctr), or Exportin-5 depleted (Xpo5KO) mES cells were subjected to 3h and 12h s4U-pulse labeling followed by total RNA extraction, alkylation, mRNA 3' end library preparation (QuantSeq, Lexogen) and high throughput sequencing.

Repositories


GEO

GSE99973

ENA

SRP109172

BioProject

PRJNA390465

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Contact


Brian Reichholf

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