|Number of samples:||18|
|Release date:||Sep 23 2017|
|Last update date:||Nov 30 2018|
|Dataset link||Thiol-linked alkylation for the metabolic sequencing of RNA [SLAM-seq in wildtype and Xpo5 knockout mES cells]|
Wildtype (wt) mouse embryonic stem (mES) cells, clonal mES cells that had been transfected with non-targeting control guide RNAs (ctr), or Exportin-5 depleted (Xpo5KO) mES cells were subjected to 3h and 12h s4U-pulse labeling followed by total RNA extraction, alkylation, mRNA 3' end library preparation (QuantSeq, Lexogen) and high throughput sequencing.
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