Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 12
Release date: Dec 21 2017
Last update date: Nov 29 2018
Access: Public
Taxon: Homo sapiens
Dataset link Genomic and proteomic resolution of heterochromatin and its restriction of alternate fate genes (RNA-seq)

Experimental Protocol


This study was designed to assess the role of human SUV39H1 and RBMX/RBMXL1 in impeding the activation of heterochromatic genes by transcription factors. RNA-seq was performed in human BJ foreskin fibroblasts treated with siRNAs in two main experimental protocols: cells expressing the hepatic transcription factors FOXA3/HNF1A/HNF4A ("hepatic TF" protocol) and cells expressing no ectopic transcription factors ("no TF" protocol). There are a total of 12 RNA-seq samples: two experimental protocols, three siRNAs per protocol, and two biological replicates per siRNA/protocol. The three siRNAs are: non-targeting control siRNA, siRNA against SUV39H1, and siRNA co-targeting RBMX and RBMX/L1

Repositories


GEO

GSE87040

ENA

SRP090039

BioProject

PRJNA343265

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