Dataset features


Application: Gene expression microarray analysis
Number of samples: 44
Release date: Jun 1 2012
Last update date: Oct 29 2018
Access: Public
Diseases: Colonic Neoplasms
Dataset link Expression data from three human DLD-1-derived colon cancer cell lines

Experimental Protocol

Three DLD-1 clonal stable cell lines created by tetracycline-regulated expression (T-REx) system were treated with or without doxycycline for RNA extraction and hybridization on Affymetrix microarrays. One of the three cell lines is a mock cell line,a parental cell line that expresses only the Tet regulator and does not overexpress any protein when induced with doxycycline (Dox). The other three cell lines can be induced by Doxycycline to overexpress wild-type dnTCF-1E or a mutant form of dnTCF-1E with five amino acid substitution mutations in the C-clamp of the E-tail, or dnLEF-1. The mock cell line and dnLEF-1 cell lines were treated with or without Dox for 23 hr. The wild-type and mutant dnTCF-1E cell lines were treated with or without Dox for 8 and 23 hr, and as well, wild-type dnTCF-1E was induced with maximal amounts of Dox (hi: 1ug/ml) for maximum expression and gene expression regulation.










Marian Waterman
Marian L. Waterman