Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 29
Release date: Jan 1 2017
Last update date: Oct 29 2018
Access: Public
Diseases: Congenital, Hereditary, and Neonatal Diseases and Abnormalities
Genes: PC
Dataset link Expression data from 0Month (New Born) Slovak Children with high PCBs exposure

Experimental Protocol


High PCB Exposed 0 month Slovak Children Extraction of total RNA was performed by using Qiagen PAXgene Blood RNA Kit IVD according to the manufacturer's instructions. Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microgram of total RNA (Expression Analysis Technical Manual, 2001, Affymetrix). Following fragmentation, 10 microgram of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array (HGU133 plus 2.0). GeneChips were washed and stained in the Affymetrix Fluidics Station 450 and scanned using the Affymetrix GeneArray Scanner 3000. The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.

Repositories


GEO

GSE32420

BioProject

PRJNA147905

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Contact


Sisir Dutta