Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Jan 29 2014
Last update date: Aug 23 2018
Access: Public
Diseases: Mucolipidoses, Multiple System Atrophy
Chemicals: Cytosine
Dataset link Expression data from primary osteoblasts isolated from calvaria of Gnptab(c.3082insC) knock-in mice compared with osteoblasts isolated from wild-type littermates

Experimental Protocol


Primary osteoblasts were isolated from calvaria of 5-day-old wild-type (WT) and MLII knock-in littermates (KI). RNA was extracted at day 10 of differentiation induced by ascorbic acid and beta-glycerophosphate and hybridization on Affymetrix microarrays. We used preparations of RNA from two individual primary cultures of osteoblasts for every genotype (WT_OB_I, WT_OB_II, KI_OB_I, KI_OB_II) and compared WT vs KI samples.

Repositories


GEO

GSE43854

ArrayExpress

E-GEOD-43854

BioProject

PRJNA187916

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