Computational protocol: Phloroglucinol Attenuates the Cognitive Deficits of the 5XFAD Mouse Model of Alzheimer’s Disease

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Protocol publication

[…] A circular water basin (120 cm in diameter) was filled with water (25 ± 2°C temperature) which was dyed with white milk, and a round platform (10 cm in diameter) was placed in the middle of one quadrant (the target quadrant). Considering the quadrant with the platform as North (N) and the other three quadrants West (W), South (S) and East (E), 4 visual cues were placed adjacent to the outer rim on the locations NW, NE, SW, SE. The animals were placed into each 3 quadrants (W, S, E) in a random order facing the wall, and was left to freely swim until they discovered the hidden platform. Mice are allowed a maximal time of 90 s to locate the platform. Finding the platform was defined as staying on it for at least 2 s. If the subject failed to find the platform, the mouse was guided and placed onto the platform for 10 s. Training to find the platform was performed for 4 consecutive days, and the whole sequence was videotaped with a digital camera attached to the ceiling. A probe test, which is a trial where the mice are placed in the basin that has the same visual cues but the platform is removed, was performed 3 days after the training was completed. The probe test was also videotaped and analyzed by Ethovision Software 8.5 (Noldus). [...] All data are expressed as the means ± standard error of the mean (SEM) values. One-way ANOVA using Tukey post-hoc comparisons (IBM SPSS Statistics 20, IL) were used to determine statistical significance. The results were considered to be statistically significant if p < 0.05. […]

Pipeline specifications

Software tools EthoVision, SPSS
Applications Miscellaneous, Macroscope & basic digital camera imaging
Organisms Mus musculus, Rattus norvegicus
Diseases Alzheimer Disease, Dementia, Mitochondrial Diseases
Chemicals Oxygen, Phloroglucinol