Computational protocol: In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons

Similar protocols

Protocol publication

[…] Gel photographs were scored using the gel image analysis software GelAnalyzer 2010a (http://www.gelanalyzer.com/). The bands were binary coded with 1 or 0 for their presence or absence in each genotype, respectively, and the coded data were subjected to statistical analysis. Estimates of similarity among all genotypes were calculated from the Jaccard’s similarity coefficient using the IBM SPSS package v. 20 (IBM Corp., New York, USA). Hierarchical cluster analysis based on the Jaccard similarity matrix with the unweighted pair group method based on arithmetic averages (UPGMA) [] was conducted using the software MEGA5 []. […]

Pipeline specifications

Software tools GelAnalyzer, MEGA
Application DNA fingerprinting