Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 24
Release date: Nov 2 2015
Last update date: Oct 24 2018
Access: Public
Diseases: Bacterial Infections, Bronchial Diseases, Haemophilus Infections
Dataset link Dual RNA-sequencing of nontypeable Haemophilus influenzae and host cell transcriptomes reveals new aspects of host-pathogen interface

Experimental Protocol


Primary human bronchial epithelium was infected with NTHi at a multiplicity of 100:1. Total RNA was isolated at 1, 6, 24 and 72 h post-infection in three biologically-independent experiments and cDNA libraries were prepared and sequenced with Illumina HiSeq 2500 sequencer. At each time point, between 60 and 180 million total reads per sample were obtained of which approximately one-third could be aligned to non-rRNA regions of the bacterial and human genomes

Repositories


GEO

GSE63900

ArrayExpress

E-GEOD-63900

ENA

SRP051182

BioProject

PRJNA270371

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Contact


Raffaele Calogero
Raffaele A Calogero

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