|Number of samples:||6|
|Release date:||Jun 1 2016|
|Last update date:||Dec 9 2016|
|Diseases:||Chondrosarcoma, Glioma, Lymphoma, Melanoma, Neoplasms, Thyroid Neoplasms, Leukemia, Myeloid, Acute|
|Dataset link||DNA methylation changes induced by overexpression of IDH1mut or treatment with 2HG in the sorted mouse bone marrow cells|
HoxA9 immortalised bone marrow cells from C57BJ/6 mice were tranduced with either empty vector or pSF91-IDH1wt-IRES-EGFP, or pSF91-IDH1mut-IRES-GFP and transplanted in irradiated recipient mice. Alternatively, HoxA9 immortalised cells transduced with empty vector transplanted mice were treated with R-2HG at dose of 1mg/day for four weeks. Four weeks after transplantation/treatment GFP+ cells were sorted from mouse bone marrow, from which total DNA was extracted and subjected to Reduced Representation Bisulfite Sequencing (RRBS) to determine the genome-wide methylation signature.