Dataset features

Specifications


Application: BS-seq analysis
Number of samples: 6
Release date: Jun 1 2016
Last update date: Dec 9 2016
Access: Public
Diseases: Chondrosarcoma, Glioma, Lymphoma, Melanoma, Neoplasms, Thyroid Neoplasms, Leukemia, Myeloid, Acute
Genes: IDH1, IDH2
Dataset link DNA methylation changes induced by overexpression of IDH1mut or treatment with 2HG in the sorted mouse bone marrow cells

Experimental Protocol


HoxA9 immortalised bone marrow cells from C57BJ/6 mice were tranduced with either empty vector or pSF91-IDH1wt-IRES-EGFP, or pSF91-IDH1mut-IRES-GFP and transplanted in irradiated recipient mice. Alternatively, HoxA9 immortalised cells transduced with empty vector transplanted mice were treated with R-2HG at dose of 1mg/day for four weeks. Four weeks after transplantation/treatment GFP+ cells were sorted from mouse bone marrow, from which total DNA was extracted and subjected to Reduced Representation Bisulfite Sequencing (RRBS) to determine the genome-wide methylation signature.

Repositories


GEO

GSE77828

ArrayExpress

E-GEOD-77828

ENA

SRP069897

BioProject

PRJNA311700

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Contact


Anuhar Chaturvedi