Computational protocol: Overexpression of OsMYB48-1, a Novel MYB-Related Transcription Factor, Enhances Drought and Salinity Tolerance in Rice

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Protocol publication

[…] The homolog genes of OsMYB48-1 were searched through BLAST database (http://www.ncbi.nlm.nih.gov/BLAST/). The phylogenetic tree was constructed in MEGA5 software based on neighbor-joining method. Numbers indicate percentage values after 1000 replications. The multiple sequence alignment was performed with ClustalW. We searched the promoter sequence (2,000 bp upstream from the transcription start site) against the PLACE database (http://www.dna.affrc.go.jp/PLACE/signalscan.html) to detect the cis-acting elements for reveal the regulatory roles of OsMYB48-1. […]

Pipeline specifications

Software tools MEGA, Clustal W
Application Phylogenetics
Organisms Oryza sativa
Chemicals Abscisic Acid, Hydrogen Peroxide, Malondialdehyde, Mannitol, Polyethylene Glycols, Sodium Chloride