Computational protocol: Historic DNA for taxonomy and conservation: A case-study of a century-old Hawaiian hawkmoth type (Lepidoptera: Sphingidae)

Similar protocols

Protocol publication

[…] To reconstruct the phylogenetic position of H. calida hawaiiensis, a maximum likelihood (ML) tree with bootstrap support values was reconstructed using RAxML [] (implemented in raxmlGUI1.5b1 []; best of 10 replicate tree searches; GTRCAT; 1000 thorough bootstrap replicates). The best partitioning scheme and evolutionary models were determined with PartitionFinder 1.1.1 [] using the AICc criterion and the greedy search algorithm (GTR+I+G; three-partition scheme COI1+COII3, COI2+COII1 and COI3+COII2). Distance comparisons were performed with MEGA5 []. […]

Pipeline specifications

Software tools RAxML, raxmlGUI, PartitionFinder, MEGA
Application Phylogenetics
Organisms Corvus hawaiiensis