Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 15
Release date: Nov 5 2012
Last update date: Oct 29 2018
Access: Public
Diseases: Granulosa Cell Tumor, Ovarian Diseases
Mutations: c|SUB|C|402|G
Computational protocol: Primer3, SLqPCR, affyQCReport, limma, GeneSetDB, IPA, GATHER
Dataset link Gene knockdown and overexpression of 402C>G FOXL2 in COV434 and KGN cells

Experimental Protocol


Mutant FOXL2 was silenced in KGN cells with the use of siRNA and a non-targetting siRNA control. Wildtype and mutant FOXL2 were overexpressed in COV434 cells with the use of overexpression plasmids and an empty plasmid control. Each experiment was performed in triplicate. Total RNA was harvested at 24h post transfection and hybridised to Affymetrix U133 plus 2.0 arrays.

Repositories


GEO

GSE39890

ArrayExpress

E-GEOD-39890

BioProject

PRJNA171996

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Contact


Roseanne Rosario

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